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by Ivano Zara with the help and advice of Riccardo Schiavon
Comments and observations are welcome. Please, write to ivano.zara.bio @ gmail.com

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DNA hybridization reaction
If you are interested in understanding, examining in detail the DNA hybridization reaction, understanding the melting temperature, what it is and how to arrive at its final equation, also, being able to calculate the fraction of hybridized ss strand as a function of temperature and arriving at its final equation by developing in detail all the easy mathematical steps.
Then here you are in the right place, click on the link DNA hybridization reaction
Please, Try the new window with launch menus.
Apparently it is not very different, but the structure is different. It will soon be default. Please let me know if you encounter any problems.
In this section, have been grouped, the software used to build the primers
using the Nearest-Neighbor thermodynamic parameters

  • Human Genomic PickPrimer (with variant)
    (create PCR Primers on regions without variants reported in various DBs)
     
  • Sequence PickPrime Sequence PickPrime
    (pick primers from a DNA sequence)
     
Some of these applications are / were also installed at Promix: CRIBI (University of Padua)
Sorry, but the CRIBI server is out of order at the moment

2019-12 Last internal database updates with files, tables or App:
- Genomic versions GRCh37/hg19 and GRCh38/hg38 available
- HGNC (Custom Download) for gene names and synonyms.
- RefGene from UCSC: To loacalize the exonic boundaries of transcripts in genomic sequences
- RefMrna from UCSC: for transcripts sequences.
- LRG_RefSeqGene from NCBI: to match the IDs of the transcripts (NM_ *) to the IDs of the proteins (NP_ *) 
     and the standard transcripts of the genes
- refLink from UCSC: as above
- App Eutils from NCBI: as above.
- dbSNPs 151 for SNPs variants from NCBI.
- For allelic frequencies: GnomAD v2.1.1 for GRCh37/hg19 (exomes and genomes), v3.0 for GRCh38/hg38 (genomes).
Note: If the standard transcripts could not be obtained with LRG_RefSeqGene, 
      then the longest transcript of the gene was used